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control cat 1122 shrna  (Vector Biolabs)


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    Structured Review

    Vector Biolabs control cat 1122 shrna
    A) Schematic diagram of region-specific downregulation of Sparc expression via injections of the AAV9 viral particles carrying scrm (control) and Sparc <t>shRNA</t> constructs. A1-2) WB analysis of the viral downregulation of Sparc expression in WT mice showed that SPARC protein enrichment in the PFC of WT mice injected with shRNA is not changed after exposure to social defeat (A1). The SPARC protein is enriched in the synaptosomal fraction of the PFC (A2), in scrm control and shRNA mice, but the shRNA mice have significantly reduced enrichment in both fractions. B1-3) Behavioral outcome of Sparc downregulation showed that Sparc shRNA injected mice have significantly decreased social interaction (B1, scrm -SD vs scrm +SD p=0.0138; scrm-SD vs Sparc shRNA+SD p=0.0303), increased latency to bite (B2, scrm -SD vs shRNA -SD p=0.0189), as well as decreased time spent in the center of the arena (B3, scrm -SD vs. shRNA +SD p=0.095, shRNA - SD vs. shRNA +SD, p=0.0159) for shRNA injected mice. C) Sparc -KO mice exhibit significantly reduced social interaction after exposure to social defeat (WT vs Sparc-KO+SD, p=0.0034; Sparc-KO -SD vs. Sparc-KO +SD, p=0.0007; WT+SD vs. Sparc-KO + SD, p=0.0110). D) RNAscope with the mouse-specific Sparc RNA probe shows that Sparc expression (yellow) level is restored in the PrL cortex of Sparc-KO mice. E) Social interaction after defeat stress is modestly improved by Sparc OE virus injection into the PFC of the Sparc -KO mice, but it does not reach statistical significance. F) Sparc -KO mice with restored Sparc expression showed statistically significant reduction in latency to bite after social defeat compared to all other groups of mice (OE +SD vs. Sparc-KO p=0.0186, vs. scrm p=0.0003, vs. scrm +SD p=0.0477, vs. OE -SD p<0.0001). G) Mice with restored Sparc expression exposed to social defeat showed statistically significant increase in time spent in the center of the arena (OE + SD vs. Sparc-KO p=0.0320, vs. scrm p=0.0027, vs. OE p=0.006).
    Control Cat 1122 Shrna, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control cat 1122 shrna/product/Vector Biolabs
    Average 96 stars, based on 60 article reviews
    control cat 1122 shrna - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Astrocyte-derived extracellular matrix proteins regulate synapse remodeling in stress-induced depression"

    Article Title: Astrocyte-derived extracellular matrix proteins regulate synapse remodeling in stress-induced depression

    Journal: bioRxiv

    doi: 10.1101/2024.12.30.630684

    A) Schematic diagram of region-specific downregulation of Sparc expression via injections of the AAV9 viral particles carrying scrm (control) and Sparc shRNA constructs. A1-2) WB analysis of the viral downregulation of Sparc expression in WT mice showed that SPARC protein enrichment in the PFC of WT mice injected with shRNA is not changed after exposure to social defeat (A1). The SPARC protein is enriched in the synaptosomal fraction of the PFC (A2), in scrm control and shRNA mice, but the shRNA mice have significantly reduced enrichment in both fractions. B1-3) Behavioral outcome of Sparc downregulation showed that Sparc shRNA injected mice have significantly decreased social interaction (B1, scrm -SD vs scrm +SD p=0.0138; scrm-SD vs Sparc shRNA+SD p=0.0303), increased latency to bite (B2, scrm -SD vs shRNA -SD p=0.0189), as well as decreased time spent in the center of the arena (B3, scrm -SD vs. shRNA +SD p=0.095, shRNA - SD vs. shRNA +SD, p=0.0159) for shRNA injected mice. C) Sparc -KO mice exhibit significantly reduced social interaction after exposure to social defeat (WT vs Sparc-KO+SD, p=0.0034; Sparc-KO -SD vs. Sparc-KO +SD, p=0.0007; WT+SD vs. Sparc-KO + SD, p=0.0110). D) RNAscope with the mouse-specific Sparc RNA probe shows that Sparc expression (yellow) level is restored in the PrL cortex of Sparc-KO mice. E) Social interaction after defeat stress is modestly improved by Sparc OE virus injection into the PFC of the Sparc -KO mice, but it does not reach statistical significance. F) Sparc -KO mice with restored Sparc expression showed statistically significant reduction in latency to bite after social defeat compared to all other groups of mice (OE +SD vs. Sparc-KO p=0.0186, vs. scrm p=0.0003, vs. scrm +SD p=0.0477, vs. OE -SD p<0.0001). G) Mice with restored Sparc expression exposed to social defeat showed statistically significant increase in time spent in the center of the arena (OE + SD vs. Sparc-KO p=0.0320, vs. scrm p=0.0027, vs. OE p=0.006).
    Figure Legend Snippet: A) Schematic diagram of region-specific downregulation of Sparc expression via injections of the AAV9 viral particles carrying scrm (control) and Sparc shRNA constructs. A1-2) WB analysis of the viral downregulation of Sparc expression in WT mice showed that SPARC protein enrichment in the PFC of WT mice injected with shRNA is not changed after exposure to social defeat (A1). The SPARC protein is enriched in the synaptosomal fraction of the PFC (A2), in scrm control and shRNA mice, but the shRNA mice have significantly reduced enrichment in both fractions. B1-3) Behavioral outcome of Sparc downregulation showed that Sparc shRNA injected mice have significantly decreased social interaction (B1, scrm -SD vs scrm +SD p=0.0138; scrm-SD vs Sparc shRNA+SD p=0.0303), increased latency to bite (B2, scrm -SD vs shRNA -SD p=0.0189), as well as decreased time spent in the center of the arena (B3, scrm -SD vs. shRNA +SD p=0.095, shRNA - SD vs. shRNA +SD, p=0.0159) for shRNA injected mice. C) Sparc -KO mice exhibit significantly reduced social interaction after exposure to social defeat (WT vs Sparc-KO+SD, p=0.0034; Sparc-KO -SD vs. Sparc-KO +SD, p=0.0007; WT+SD vs. Sparc-KO + SD, p=0.0110). D) RNAscope with the mouse-specific Sparc RNA probe shows that Sparc expression (yellow) level is restored in the PrL cortex of Sparc-KO mice. E) Social interaction after defeat stress is modestly improved by Sparc OE virus injection into the PFC of the Sparc -KO mice, but it does not reach statistical significance. F) Sparc -KO mice with restored Sparc expression showed statistically significant reduction in latency to bite after social defeat compared to all other groups of mice (OE +SD vs. Sparc-KO p=0.0186, vs. scrm p=0.0003, vs. scrm +SD p=0.0477, vs. OE -SD p<0.0001). G) Mice with restored Sparc expression exposed to social defeat showed statistically significant increase in time spent in the center of the arena (OE + SD vs. Sparc-KO p=0.0320, vs. scrm p=0.0027, vs. OE p=0.006).

    Techniques Used: Expressing, Control, shRNA, Construct, Protein Enrichment, Injection, RNAscope, Virus



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    control cat 1122 shrna  (Vector Biolabs)
    96
    Vector Biolabs control cat 1122 shrna
    A) Schematic diagram of region-specific downregulation of Sparc expression via injections of the AAV9 viral particles carrying scrm (control) and Sparc <t>shRNA</t> constructs. A1-2) WB analysis of the viral downregulation of Sparc expression in WT mice showed that SPARC protein enrichment in the PFC of WT mice injected with shRNA is not changed after exposure to social defeat (A1). The SPARC protein is enriched in the synaptosomal fraction of the PFC (A2), in scrm control and shRNA mice, but the shRNA mice have significantly reduced enrichment in both fractions. B1-3) Behavioral outcome of Sparc downregulation showed that Sparc shRNA injected mice have significantly decreased social interaction (B1, scrm -SD vs scrm +SD p=0.0138; scrm-SD vs Sparc shRNA+SD p=0.0303), increased latency to bite (B2, scrm -SD vs shRNA -SD p=0.0189), as well as decreased time spent in the center of the arena (B3, scrm -SD vs. shRNA +SD p=0.095, shRNA - SD vs. shRNA +SD, p=0.0159) for shRNA injected mice. C) Sparc -KO mice exhibit significantly reduced social interaction after exposure to social defeat (WT vs Sparc-KO+SD, p=0.0034; Sparc-KO -SD vs. Sparc-KO +SD, p=0.0007; WT+SD vs. Sparc-KO + SD, p=0.0110). D) RNAscope with the mouse-specific Sparc RNA probe shows that Sparc expression (yellow) level is restored in the PrL cortex of Sparc-KO mice. E) Social interaction after defeat stress is modestly improved by Sparc OE virus injection into the PFC of the Sparc -KO mice, but it does not reach statistical significance. F) Sparc -KO mice with restored Sparc expression showed statistically significant reduction in latency to bite after social defeat compared to all other groups of mice (OE +SD vs. Sparc-KO p=0.0186, vs. scrm p=0.0003, vs. scrm +SD p=0.0477, vs. OE -SD p<0.0001). G) Mice with restored Sparc expression exposed to social defeat showed statistically significant increase in time spent in the center of the arena (OE + SD vs. Sparc-KO p=0.0320, vs. scrm p=0.0027, vs. OE p=0.006).
    Control Cat 1122 Shrna, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control cat 1122 shrna/product/Vector Biolabs
    Average 96 stars, based on 1 article reviews
    control cat 1122 shrna - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Schematic diagram of region-specific downregulation of Sparc expression via injections of the AAV9 viral particles carrying scrm (control) and Sparc shRNA constructs. A1-2) WB analysis of the viral downregulation of Sparc expression in WT mice showed that SPARC protein enrichment in the PFC of WT mice injected with shRNA is not changed after exposure to social defeat (A1). The SPARC protein is enriched in the synaptosomal fraction of the PFC (A2), in scrm control and shRNA mice, but the shRNA mice have significantly reduced enrichment in both fractions. B1-3) Behavioral outcome of Sparc downregulation showed that Sparc shRNA injected mice have significantly decreased social interaction (B1, scrm -SD vs scrm +SD p=0.0138; scrm-SD vs Sparc shRNA+SD p=0.0303), increased latency to bite (B2, scrm -SD vs shRNA -SD p=0.0189), as well as decreased time spent in the center of the arena (B3, scrm -SD vs. shRNA +SD p=0.095, shRNA - SD vs. shRNA +SD, p=0.0159) for shRNA injected mice. C) Sparc -KO mice exhibit significantly reduced social interaction after exposure to social defeat (WT vs Sparc-KO+SD, p=0.0034; Sparc-KO -SD vs. Sparc-KO +SD, p=0.0007; WT+SD vs. Sparc-KO + SD, p=0.0110). D) RNAscope with the mouse-specific Sparc RNA probe shows that Sparc expression (yellow) level is restored in the PrL cortex of Sparc-KO mice. E) Social interaction after defeat stress is modestly improved by Sparc OE virus injection into the PFC of the Sparc -KO mice, but it does not reach statistical significance. F) Sparc -KO mice with restored Sparc expression showed statistically significant reduction in latency to bite after social defeat compared to all other groups of mice (OE +SD vs. Sparc-KO p=0.0186, vs. scrm p=0.0003, vs. scrm +SD p=0.0477, vs. OE -SD p<0.0001). G) Mice with restored Sparc expression exposed to social defeat showed statistically significant increase in time spent in the center of the arena (OE + SD vs. Sparc-KO p=0.0320, vs. scrm p=0.0027, vs. OE p=0.006).

    Journal: bioRxiv

    Article Title: Astrocyte-derived extracellular matrix proteins regulate synapse remodeling in stress-induced depression

    doi: 10.1101/2024.12.30.630684

    Figure Lengend Snippet: A) Schematic diagram of region-specific downregulation of Sparc expression via injections of the AAV9 viral particles carrying scrm (control) and Sparc shRNA constructs. A1-2) WB analysis of the viral downregulation of Sparc expression in WT mice showed that SPARC protein enrichment in the PFC of WT mice injected with shRNA is not changed after exposure to social defeat (A1). The SPARC protein is enriched in the synaptosomal fraction of the PFC (A2), in scrm control and shRNA mice, but the shRNA mice have significantly reduced enrichment in both fractions. B1-3) Behavioral outcome of Sparc downregulation showed that Sparc shRNA injected mice have significantly decreased social interaction (B1, scrm -SD vs scrm +SD p=0.0138; scrm-SD vs Sparc shRNA+SD p=0.0303), increased latency to bite (B2, scrm -SD vs shRNA -SD p=0.0189), as well as decreased time spent in the center of the arena (B3, scrm -SD vs. shRNA +SD p=0.095, shRNA - SD vs. shRNA +SD, p=0.0159) for shRNA injected mice. C) Sparc -KO mice exhibit significantly reduced social interaction after exposure to social defeat (WT vs Sparc-KO+SD, p=0.0034; Sparc-KO -SD vs. Sparc-KO +SD, p=0.0007; WT+SD vs. Sparc-KO + SD, p=0.0110). D) RNAscope with the mouse-specific Sparc RNA probe shows that Sparc expression (yellow) level is restored in the PrL cortex of Sparc-KO mice. E) Social interaction after defeat stress is modestly improved by Sparc OE virus injection into the PFC of the Sparc -KO mice, but it does not reach statistical significance. F) Sparc -KO mice with restored Sparc expression showed statistically significant reduction in latency to bite after social defeat compared to all other groups of mice (OE +SD vs. Sparc-KO p=0.0186, vs. scrm p=0.0003, vs. scrm +SD p=0.0477, vs. OE -SD p<0.0001). G) Mice with restored Sparc expression exposed to social defeat showed statistically significant increase in time spent in the center of the arena (OE + SD vs. Sparc-KO p=0.0320, vs. scrm p=0.0027, vs. OE p=0.006).

    Article Snippet: Adeno-associated viral particles, serotype AAV9 carrying the shRNA vector against mouse Sparc (cat # shAAV-272934) or scrambled control (cat # 1122) shRNA were purchased from Vector Biolabs.

    Techniques: Expressing, Control, shRNA, Construct, Protein Enrichment, Injection, RNAscope, Virus